IJMEG Copyright © 2010-present. All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Mol Epidemiol Genet 2010;1(1):67-73.

Original Article
The -590C/T IL4 single-nucleotide polymorphism as a
genetic factor of atopic allergy

Roldan M. de Guia, John Donnie A. Ramos

Department of Biochemistry, Faculty of Pharmacy; 2Department of Biological Sciences, College of Science; and
Research Center for the Natural Sciences, University of Santo Tomas, Espana Blvd., Manila, Philippines.

Received September 22, 2009; accepted November 20, 2009; available online November 25, 2009

Abstract: Elevated IgE levels in individuals with asthma, allergic rhinitis, and atopic dermatitis represents a situation in that
increased IL4 production seems to occur because of the genetic component of the disease. In this study, one-hundred two
matched-pairs of allergic and non-allergic individuals were phenotyped for total serum IgE level using enzyme-linked
immunosorbent assay (ELISA). Atopic status was defined by serum IgE concentration ≥100 IU/mL. The -590C/T IL4
(rs2243250) was screened by polymerase chain reaction-restriction fragmentlength polymorphism (PCR-RFLP) analysis. An
association between the IL4 -590 TT genotype and levels of IgE was confirmed in the study population (ANOVA p=0.017).
Furthermore, the IL4 T allele was significantly increased in allergic (0.299) compared with non-allergic subjects (0.172) (OR=2.
060, 95% CI=1.285-3.301, 2 uncorrected p=0.002) at total serum IgE cut-off of 100 IU/mL. A significant relationship between
IL4 -590 TT genotype and very high IgE levels (>1000 IU/mL) (OR=3.968, 95% CI=1.499-10.5, 2 uncorrected p=0.01624) was
also established. The -590C/T IL4 polymorphism is a potential risk factor to and correlates with atopic allergy.(IJMEG909002).

Key words: CYP7A1, polymorphisms, proximal colon cancer, Japanese

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Address all correspondence to:
Roldan M. de Guia, PhD
The Department of Biochemistry
Faculty of Pharmacy, University of Santo Tomas
Espana Blvd., Manila, 1008, Philippines
Tel: (632) 731-4040
rmdeguia@mnl.ust.edu.ph, or wgdequincey@yahoo.com