Original Article The -590C/T IL4 single-nucleotide polymorphism as a genetic factor of atopic allergy
Roldan M. de Guia, John Donnie A. Ramos
Department of Biochemistry, Faculty of Pharmacy; 2Department of Biological Sciences, College of Science; and Research Center for the Natural Sciences, University of Santo Tomas, Espana Blvd., Manila, Philippines.
Received September 22, 2009; accepted November 20, 2009; available online November 25, 2009
Abstract: Elevated IgE levels in individuals with asthma, allergic rhinitis, and atopic dermatitis represents a situation in that increased IL4 production seems to occur because of the genetic component of the disease. In this study, one-hundred two matched-pairs of allergic and non-allergic individuals were phenotyped for total serum IgE level using enzyme-linked immunosorbent assay (ELISA). Atopic status was defined by serum IgE concentration ≥100 IU/mL. The -590C/T IL4 (rs2243250) was screened by polymerase chain reaction-restriction fragmentlength polymorphism (PCR-RFLP) analysis. An association between the IL4 -590 TT genotype and levels of IgE was confirmed in the study population (ANOVA p=0.017). Furthermore, the IL4 T allele was significantly increased in allergic (0.299) compared with non-allergic subjects (0.172) (OR=2. 060, 95% CI=1.285-3.301, 2 uncorrected p=0.002) at total serum IgE cut-off of 100 IU/mL. A significant relationship between IL4 -590 TT genotype and very high IgE levels (>1000 IU/mL) (OR=3.968, 95% CI=1.499-10.5, 2 uncorrected p=0.01624) was also established. The -590C/T IL4 polymorphism is a potential risk factor to and correlates with atopic allergy.(IJMEG909002).
Key words: CYP7A1, polymorphisms, proximal colon cancer, Japanese
Address all correspondence to: Roldan M. de Guia, PhD The Department of Biochemistry Faculty of Pharmacy, University of Santo Tomas Espana Blvd., Manila, 1008, Philippines Tel: (632) 731-4040 E-mail: firstname.lastname@example.org, or email@example.com