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Int J Mol Epidemiol Genet 2013;4(4):207-217

Original Article
CYP2E1 and NQO1 genotypes and bladder cancer risk in a Lebanese population

Hussein A Basma, Loulou H Kobeissi, Michel E Jabbour, Mohamad A Moussa, Hassan R Dhaini

Faculty of Health Sciences, University of Balamand, Beirut, Lebanon; Mel and Enid Zuckerman College of Public Health,
University of Arizona, Tucson, USA; Department of Urology, St George Hospital University Medical Center, Beirut, Lebanon;
Division of Urology, Bahman Hospital, Beirut, Lebanon

Received November 4, 2013; Accepted November 19, 2013; Epub November 28, 2013; Published December 15, 2013

Abstract: Urinary bladder cancer incidence in Lebanon ranks among the highest in the world. Cytochrome P450 2E1
(CYP2E1), NAD(P)H quinone oxidoreductase1 (NQO1), and N-Acetyltransferase1 (NAT1), are drug-metabolizing enzymes
(DMEs) involved in the metabolism of carcinogens, such as arylamines and heterocyclic amines, implicated in bladder cancer.
The present study attempts to investigate the role of these DMEs genetic polymorphism in bladder cancer risk among
Lebanese men. 54 cases and 106 controls were recruited from two hospitals in Beirut. An interview-based questionnaire was
administered to assess suspected environmental and occupational risk factors. PCR-RFLP was performed on blood-based
DNA samples to determine DMEs genotypes. Associations between bladder cancer and putative risk factors were measured
using adjusted odds ratios (ORs) and their 95% confidence intervals (CIs). Results showed CYP2E1 c1/c1, NAT1*14A, and
smoking, to be risk factors for bladder cancer. No significant differences in frequency distribution of the NQO1 genotypes were
found in cases versus controls. The odds of carrying the CYP2E1 c1/c1 genotype were 4 times higher in cases compared to
controls (OR=3.97, 95% CI: 0.48-32.7). The odds of carrying at least one NAT1*14A allele were 14 times higher in cases
versus controls (OR= 14.4, 95% CI: 1.016-204.9). Our study suggests CYP2E1 c1/c1, NAT1*14A, and smoking, as potential risk
factors for bladder cancer in Lebanese. Further studies with larger samples must be conducted to confirm these findings.

Keywords: Cytochrome P450 CYP2E1, NQO1, N-Acetyltransferase NAT1, bladder cancer, Lebanese

Address correspondence to: Dr. Hassan R Dhaini, The Faculty of Health Sciences, University of Balamand, P.O. Box 166378
Ashrafieh, Beirut, Lebanon. E-mail: hassan.dhaini@balamand.edu.lb