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Int J Mol Epidemiol Genet 2012;3(2):144-152

Original Article
Characterization of human herpes virus 8 genotypes in Kaposi's sarcoma
patients in Tehran, Iran

Kayhan Azadmanesh, Zeinab Sadat Norouzfar, Amir Sohrabi, Zahra Safaie-Naraghi, Afshin Moradi, Parichehre Yaghmaei,
Mona Masoumeh Naraghi, Arash Arashkia, Ali Eslamifar

Department of Virology, Pasteur Institute of Iran, Tehran, Iran; Department of Biology, Science and Research Branch, Islamic
Azad University, Tehran, Iran; Department of Molecular Medicine, School of Advanced Medical Technologies, Tehran University
of Medical Sciences, Tehran, Iran; Department of Pathology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran;
Department of Cancer Researches, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Tehran University of Medical
Sciences, Tehran, Iran; Department of Clinical Researches, Pasteur Institute of Iran, Tehran, Iran

Received April 3, 2012; accepted May 1, 2012; Epub May 15, 2012; Published May 30, 2012

Abstract: Kaposi's sarcoma (KS) associated with HHV8 is one of the common connective tissue malignancies especially in
immunocompromised patients. The aim of the present study was to determine the common HHV8 genotypes in hospitalized
patients in Tehran, Iran. A total of 36 archival paraffin-embedded KS tissue samples of patients with common characterization
of KS were collected between 1999 to 2010 from hospitals in Tehran, Iran. After identifying the presence of HHV8 by
amplification of its ORF 26 region, the ORF K1 region was amplified, sequenced and used for phylogenetic analysis. Among 30
ORF 26 positive cases, ORF K1 was amplified successfully in 14 cases. Consistent with other studies in Asia, subtype A (9
cases; 64.28%) and subtype C (5 cases; 35.71%) were detected by phylogenetic analysis. This result is in concordance with
results from other countries of the region, however the ratio of genotype A to C is higher in our study compared to another study
in the country. (IJMEG1204002).

Keywords: Kaposi's sarcoma, HHV8, PCR, Tehran


Address all correspondence to:
Dr. Azadmanesh K
P.O. Box 1316942551, Department of Virology
Pasteur Institute of Iran- 12th Farvardin St
Pasteur Ave, Tehran, Iran.
Tel: +98-2166496682; Fax: +98-2166496682
E-mail: azadmanesh@pasteur.ac.ir

Dr. Eslamifar A
P.O. Box 1316942551, Department of Clinical researches
Pasteur Institute of Iran, 2th Farvardin St
Pasteur Ave, Tehran, Iran.
Tel: +98-2166953311; Fax: +98-2166465132
E-mail: info@shafaghlab.com