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Int J Mol Epidemiol Genet 2011;2(3):228-235

Original Article
Emergence of unique variants and inter-genotype recombinants of human
astroviruses infecting infants, children and adults in Kolkata, India

Madhu S. Pativada, Debarati Chatterjee, Nataraju S. Mariyappa, Krishnan Rajendran, Mihir K. Bhattacharya, Mrinmoy Ghosh,
Nobumichi Kobayashi, Triveni Krishnan

Division of Virology, Division of Data Management, Division of Clinical Medicine, National Institute of Cholera and Enteric
Diseases (NICED), P-33. C.I.T. Road, Scheme-XM, Beliaghata, Kolkata-700010, West Bengal, India; Infectious Diseases and
Beliaghata General Hospital, Kolkata 700010; Department of Hygiene, Sapporo Medical University School of Medicine, S1W17,
Chuo-ku, Sapporo 060-8556, Japan.

Received April 22, 2011; accepted May 26, 2011; Epub June 5, 2011; published August 30, 2011

Abstract: Two conserved genomic fragments viz. 289bp of ORF1a and 449bp of ORF2 amplified by RT-PCR showed
emergence of interesting recombinant strains representing new and novel genetic variants (n=5) within eight different
genotypes of astroviruses known to date. HAstV-positive cases with ORF1a [HAstV genotype G2 or G8] and ORF2 [HAstV
genotype G1, G2, or G3] were detected as sole or mixed infection among infants, children and adults in Kolkata with severe
illness owing to acute gastroenteritis that required hospitalization for treatment between 2007 and 2009. The twelve interesting
recombinants were of type HAstV _ ORF1a _ ORF2 as HAstV _ G8_ G2 (n=1), HAstV _ G8_ G1 (n=10) and HAstV _ G2_ G3
(n=1). (IJMEG1104002).

Key words: Human astrovirus inter-genotype recombinants, unique emerging variants for ORF1a and ORF2, acute
gastroenteritis, genotyping

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Address all correspondence to:
Dr Triveni Krishnan
Division of Virology, National Institute of Cholera & Enteric Diseases (NICED),
P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata, PIN 700010, West Bengal, India.
Tel: 91-33-23633852
Fax: 91-33-23632398
E-mail:drtriveni.krishnan@gmail.com